Amount funded: $20,000
PI: Kateryna Makova, Department of Biology, Eberly College of Science
Co-I: Mark D. Shriver, Department of Anthropology, College of the Liberal Arts
Co-I: David A. Puts, Department of Anthropology, College of the Liberal Arts
Co-I: Michael DeGiorgio, Department of Biology, Eberly College of Science
Co-I: Paul Medvedev, Department of Computer Science and Engineering, College of Engineering, and Dept. of Biochemistry and Molecular Biology, Eberly College of Science
Co-I: Francesca Chiaromonte, Department of Statistics, Eberly College of Science
The goal of the project is to study the variation of ampliconic gene copy number in 100 human males representing the major Y-chromosomal haplogroups; 5 human males per each of 20 Y-haplogroup assayed will be studied. Ampliconic genes are male fertility genes present in multiple copies on the Y chromosome. They are important in reproduction, especially in fertility and spermatogenesis. An undergraduate student is performing Droplet Digital PCR (ddPCR) copy number assays of 9 ampliconic gene families found on the Y chromosome to determine their sizes in each individual.